Development of Point of Care Device/system for in-situ Blood Transfusion
About this project
This project is to develop a novel device that can effectively capture and release platelets from blood samples. The device will combine two advanced technologies that includes nanoyeast based capturing and Electro-hemodynamics induced release of captured platelets. Platelets can adhere to several adhesion molecules that allow a platelet to be captured. The nanoyeast based capturing system will be designed using naturally existing adhesion molecules such as Collagen, Fibrinogen and vWF protein. The adhesion proteins installed nanoyeast can bind to glycoprotein IV, glycoprotein VI and integrin ?2?1, integrin ?IIb?3, glycoprotein Ib? receptor on the platelets surface. The nanoyeast captured platelets make non-covalent interactions with the adhesive proteins and it is possible to release them through “electro-hemodynamics” induced nanoshear forces.
The specific aims are:
Design and Engineering of platelet adhesive proteins for Nanoyeast system
Implementation of Electro-hemodynamics for platelet release from adhesive-Nanoyeast
Integrate the developed adhesive-Nanoyeast and Electro-hemodynamic systems into a blood-bag device
This research work includes gene synthesis, recombinant protein production and purification, yeast surface display, flow cytometry, and electro-hydrodynamic analysis.
This bioengineered device could be effectively used for selective capture and controlled release of platelets from the blood. The work shall have a tremendous role in in-situ platelet transfusion for trauma patients, battlefields as well as in space.
Information for applicants
Student should have the zeal to excel in research, motivated and hardworking, given that the Ph.D. must be completed within 4 years including coursework and exchange visit.
Should have hands on experience with biochemistry, molecular biology, separation chemistry, chromatography etc., along with good understanding of microfluidics and cell biology/cell culture etc.
Expected qualifications (Course/Degrees etc.)
M.Sc./M.tech./ MBBS/ or equivalent with Biochemistry/Biotechnology/ Chemistry/ Cell biology/ material science or allied disciplines.