The role of lipid composition in the broad-spectrum activity of antimicrobial peptides

Project description

Antimicrobial peptides (AMPs) have independently evolved several times in different organisms and form part of most animals’ innate immune systems. The main mechanism by which AMPs exert their cell-killing activity is by disruption of cellular membranes and subsequently interfering with a wide range of processes critical for cellular functions.

Some AMPs have remarkable broad-spectrum activity and show antibacterial, antifungal, antiviral, and antiparasitic activity despite the varying lipid compositions and structures of membranes and cell walls in these organisms. Also, compared to the antibacterial activities of AMPs, much less is known about their activities against parasites

This project aims to investigate the molecular basis that governs the broad-spectrum activity of selected AMPs. Specifically, the project will investigate how complex lipid composition contributes to broad-spectrum activity. The investigated peptides show activity against several fungal and bacterial pathogens, as well as Leishmania and Trypanosoma cruzi (the causative agent of Chagas disease).

The project will combine cell-based and biophysical experiments with biomolecular simulation approaches to study how the lipid composition of complex membranes and the physical structure of membranes and cell walls dictate the peptides’ membrane-altering activities and their cellular uptake.

Outcomes

Training and knowledge in biochemistry, biomedical sciences, biotechnology and/or chemistry. Good quantitative analysis skills and interest in computational approaches

Essential capabilities

Training and knowledge in biochemistry, biomedical sciences, biotechnology and/or chemistry. Good quantitative analysis skills and interest in computational approaches

Desireable capabilities

Prior experience in peptide biochemistry

Expected qualifications (Course/Degrees etc.)

For i-students: Master’s or equivalent in biochemistry/chemistry/biotechnology with a thesis/research component. For q-students:, 1st class Honours in biochemistry/chemistry/biotechnology